A consensus linkage map of lentil based on DArT markers from three RIL mapping populations

نویسندگان

  • Duygu Ates
  • Secil Aldemir
  • Ahmad Alsaleh
  • Semih Erdogmus
  • Seda Nemli
  • Abdullah Kahriman
  • Hakan Ozkan
  • Albert Vandenberg
  • Bahattin Tanyolac
چکیده

BACKGROUND Lentil (Lens culinaris ssp. culinaris Medikus) is a diploid (2n = 2x = 14), self-pollinating grain legume with a haploid genome size of about 4 Gbp and is grown throughout the world with current annual production of 4.9 million tonnes. MATERIALS AND METHODS A consensus map of lentil (Lens culinaris ssp. culinaris Medikus) was constructed using three different lentils recombinant inbred line (RIL) populations, including "CDC Redberry" x "ILL7502" (LR8), "ILL8006" x "CDC Milestone" (LR11) and "PI320937" x "Eston" (LR39). RESULTS The lentil consensus map was composed of 9,793 DArT markers, covered a total of 977.47 cM with an average distance of 0.10 cM between adjacent markers and constructed 7 linkage groups representing 7 chromosomes of the lentil genome. The consensus map had no gap larger than 12.67 cM and only 5 gaps were found to be between 12.67 cM and 6.0 cM (on LG3 and LG4). The localization of the SNP markers on the lentil consensus map were in general consistent with their localization on the three individual genetic linkage maps and the lentil consensus map has longer map length, higher marker density and shorter average distance between the adjacent markers compared to the component linkage maps. CONCLUSION This high-density consensus map could provide insight into the lentil genome. The consensus map could also help to construct a physical map using a Bacterial Artificial Chromosome library and map based cloning studies. Sequence information of DArT may help localization of orientation scaffolds from Next Generation Sequencing data.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

SNP-Based Linkage Mapping for Validation of QTLs for Resistance to Ascochyta Blight in Lentil

Lentil (Lens culinaris Medik.) is a self-pollinating, diploid, annual, cool-season, food legume crop that is cultivated throughout the world. Ascochyta blight (AB), caused by Ascochyta lentis Vassilievsky, is an economically important and widespread disease of lentil. Development of cultivars with high levels of durable resistance provides an environmentally acceptable and economically feasible...

متن کامل

A High Density Consensus Map of Rye (Secale cereale L.) Based on DArT Markers

BACKGROUND Rye (Secale cereale L.) is an economically important crop, exhibiting unique features such as outstanding resistance to biotic and abiotic stresses and high nutrient use efficiency. This species presents a challenge to geneticists and breeders due to its large genome containing a high proportion of repetitive sequences, self incompatibility, severe inbreeding depression and tissue cu...

متن کامل

Novel SSR Markers from BAC-End Sequences, DArT Arrays and a Comprehensive Genetic Map with 1,291 Marker Loci for Chickpea (Cicer arietinum L.)

Chickpea (Cicer arietinum L.) is the third most important cool season food legume, cultivated in arid and semi-arid regions of the world. The goal of this study was to develop novel molecular markers such as microsatellite or simple sequence repeat (SSR) markers from bacterial artificial chromosome (BAC)-end sequences (BESs) and diversity arrays technology (DArT) markers, and to construct a hig...

متن کامل

A QTL linkage map of safflower for yield under drought stress at reproductive stage

 This study reports QTL mapping for seed yield and its components in safflower genome under drought stress. The F3 families derived from the cross Mex.22-191 (tolerant) × IL.111 (sensitive) were evaluated for agronomic traits in safflower. Drought tolerance was evaluated during 10% of the flowering stage. To identify QTLs underlying tolerance to drought, mapping quantitative trait loci (QTLs) w...

متن کامل

Linkage Map Construction for Silkworm (Bombyx mori L.) Based on Amplified Fragment Length Polymorphism Markers

The domesticated silkworm, Bombyx mori, is of high commercial importance as a silk producer and is also widely used for implementation of basic and applied research. It is important to understand its genome organization using molecular markers for genetic studies and for breeding purposes. In this study, a genetic linkage map using 204 amplified fragment length polymorphism (AFLP) markers was d...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 13  شماره 

صفحات  -

تاریخ انتشار 2018